Fertilization in brown algae. I. SEM and other observations on Fucus serratus.
نویسندگان
چکیده
The cell wall secreted immediately following sperm entry into an egg can be visualized by the fluorescent dye Calcofluor white. Cell wall secretion precedes nuclear fusion by 10-20 min. SEM observations of the surface of unfertilized and fertilized eggs and sperm attachment to eggs are described. These results are discussed in relation to fertilization in sea urchins and the biochemical phenomena associated with egg-sperm recognition in Fucus.
منابع مشابه
Activators and inhibitors of the motility of Fucus Serratus
Fucus serratus is a common brown algae which occurs all round the British coast. At low tide male and female plants release antheridia and oogonia into the seawater (1) and fertilization occurs as the tide rises causing release of the encapsulated spermatozoa and eggs. In contrast to mammalian sperm which have been widely studied very little is known about the mechanism of activation of the spe...
متن کاملFertilization in brown algae. II. Evidence for lectin-sensitive complementary receptors involved in gamete recognition in Fucus serratus.
Fertilization in Fucus serratus is directly proportional to the number of sperm added, saturating at approximately 250 sperm per egg with an apparent Km of 120 sperm per egg. The effect of a range of lectins on fertilization has been tested. Preincubation of gametes with Con A and fucose-binding protein (FBP) inhibited fertilization. At low concentrations this was by specifically binding to egg...
متن کاملFertilization in brown algae. III. Preliminary characterization of putative gamete receptors from eggs and sperm of Fucus serratus.
Membrane fractions have been isolated from eggs of Fucus serrratus which inhibit fertilization in a species-specific manner. This activity is destroyed by alpha-fucosidase and alpha-mannosidase. Some 6% of the protein of this membrane fraction binds to Con A-agarose, following SDS solubilization, and when eluted with alpha-methyl mannoside inhibits fertilization when preincubated with sperm, bu...
متن کاملCa2+ signals coordinate zygotic polarization and cell cycle progression in the brown alga Fucus serratus.
Zygotes of the fucoid brown algae provide excellent models for addressing fundamental questions about zygotic symmetry breaking. Although the acquisition of polarity is tightly coordinated with the timing and orientation of the first asymmetric division--with zygotes having to pass through a G1/S-phase checkpoint before the polarization axis can be fixed--the mechanisms behind the interdependen...
متن کاملStage-dependent sensitivity to ultraviolet radiation in zygotes of the brown alga Fucus serratus.
Sensitivity to ultraviolet (UV) radiation (UV-A, lambda = 315-400 nm; plus UV-B, lambda = 280-315 nm) of zygotes of the brown alga Fucus serratus L. (Phaeophyta) has been assessed through effects on growth of developing germlings. Different stages of development were distinguished by considering 5 h periods of time after fertilisation. Both the stage of the zygote and the UV radiation condition...
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ورودعنوان ژورنال:
- Journal of cell science
دوره 32 شماره
صفحات -
تاریخ انتشار 1978